Enhanced Detection of Lipid Transfer Protein Activity by Resonance Energy Transfer with Tetramethyl-Bodipy Labeled Lipids

Resonance energy transfer assay of protein-mediated lipid transfer between vesicles.

Cytochrome c-lipid interactions: new insights from resonance energy transfer.

Resonance energy transfer (RET) from anthrylvinyl-labeled phosphatidylcholine (AV-PC) or cardiolipin (AV-CL) to cytochrome c (cyt c) heme moiety was employed to assess the molecular-level details of protein interactions with lipid bilayers composed of PC with 2.5 (CL2.5), 5 (CL5), 10 (CL10), or 20 (CL20) mol % CL under conditions of varying ionic strength and lipid/protein molar ratio. Monte Ca...

Fluorescence resonance energy transfer between quantum dot donors and dye-labeled protein acceptors.

We used luminescent CdSe-ZnS core-shell quantum dots (QDs) as energy donors in fluorescent resonance energy transfer (FRET) assays. Engineered maltose binding protein (MBP) appended with an oligohistidine tail and labeled with an acceptor dye (Cy3) was immobilized on the nanocrystals via a noncovalent self-assembly scheme. This configuration allowed accurate control of the donor-acceptor separa...

کلون کردن ژن ns-ltp (non-specific lipid-transfer protein) برنج با برچسب gst به داخل باکتری اشرشیا کلی

پروتئین های ناقل لیپید (ltps) گروهی از پروتئین ها با وزن ملکولی متوسط در نزد گیاهان هستند که دارای پتانسیل های کاربردی به ویژه در سیستم های رها سازی دارو می باشند. هدف از این تحقیق، کلون کردن ژن ltp2 مربوط به گیاه برنج(oryza sativa) در وکتور بیانی pgex-6p-2، در باکتری e.coli (top10) جهت تولید پروتئین به صورت متصل با gst می باشد. از آنجائیکه ژن ltp2 در گیاه برنج را فاقد اینترون است این ژن با کمک...

Ultra-sensitive detection of prion protein with a long range resonance energy transfer strategy.

An ultra-sensitive detection strategy for prion protein is proposed based on the long range resonance energy transfer (LrRET) from quantum dots (QDs) to the surface of gold nanoparticles (AuNPs), in which process energy donor-acceptor separation distance ranges from 9 to 22 nm.